Journal:

Article Title: Hybridization Analysis of Chesapeake Bay Virioplankton †

doi:

Figure Lengend Snippet: Flow chart of methods used in the hybridization analysis of Chesapeake Bay virioplankton.

Article Snippet: Virioplankton plugs and plugs containing phage lambda concatamers (Promega, Madison, Wis.), serving as molecular size markers, were placed into wells of a 1% SeaKem GTG agarose (FMC) gel with an overlay of molten 1% agarose.

Techniques: Hybridization

Journal:

Article Title: Hybridization Analysis of Chesapeake Bay Virioplankton †

doi:

Figure Lengend Snippet: Virioplankton probes used in this study

Article Snippet: Virioplankton plugs and plugs containing phage lambda concatamers (Promega, Madison, Wis.), serving as molecular size markers, were placed into wells of a 1% SeaKem GTG agarose (FMC) gel with an overlay of molten 1% agarose.

Techniques: Sequencing

Journal:

Article Title: Hybridization Analysis of Chesapeake Bay Virioplankton †

doi:

Figure Lengend Snippet: Autoradiograms and hybridization intensity data. DNA from the rectangle in gel A was radiolabeled and hybridized against virioplankton PFGE fingerprints of Chesapeake Bay water samples. (A) Virioplankton PFGE of water samples from station 818. Lanes: 1, lambda marker; 2, August 1995; 3 to 5, May, June, and July 1996, respectively. (B to G) Autoradiograms of virioplankton PFGE fingerprints of water samples collected at stations 908, 858, 845, 818, 744, and 724, respectively. Lanes 1 to 3, May, June, and July 1996, respectively. The box in gel A outlines the subregion from which probe DNA was harvested. *, water sample from which the probe was generated.

Article Snippet: Virioplankton plugs and plugs containing phage lambda concatamers (Promega, Madison, Wis.), serving as molecular size markers, were placed into wells of a 1% SeaKem GTG agarose (FMC) gel with an overlay of molten 1% agarose.

Techniques: Hybridization, Marker, Generated

Journal:

Article Title: Hybridization Analysis of Chesapeake Bay Virioplankton †

doi:

Figure Lengend Snippet: Autoradiograms and hybridization data obtained by using RAPD-PCR-generated probe RAPD E. (A to F) Autoradiograms of virioplankton PFGE fingerprints of water samples collected at stations 908, 858, 845, 818, 744, and 724, respectively. Lanes 1 to 3, May, June, and July 1996, respectively. *, water sample from which the probe was generated.

Article Snippet: Virioplankton plugs and plugs containing phage lambda concatamers (Promega, Madison, Wis.), serving as molecular size markers, were placed into wells of a 1% SeaKem GTG agarose (FMC) gel with an overlay of molten 1% agarose.

Techniques: Hybridization, Generated

Journal:

Article Title: Hybridization Analysis of Chesapeake Bay Virioplankton †

doi:

Figure Lengend Snippet: Autoradiograms of virioplankton PFGE fingerprints probed with RAPD 10 and RAPD 7. Probes were generated from virioplankton DNA of the water sample from station 724 in July 1996. (Series I) Southern blots probed with RAPD 10. (Series II) Southern blots probed with RAPD 7. (A to F) Water samples from stations 908, 858, 845, 818, 744, and 724, respectively. Lanes 1 to 3, May, June, and July 1996 samples, respectively.

Article Snippet: Virioplankton plugs and plugs containing phage lambda concatamers (Promega, Madison, Wis.), serving as molecular size markers, were placed into wells of a 1% SeaKem GTG agarose (FMC) gel with an overlay of molten 1% agarose.

Techniques: Generated

Journal:

Article Title: Hybridization Analysis of Chesapeake Bay Virioplankton †

doi:

Figure Lengend Snippet: Autoradiograms and virioplankton PFGE fingerprints of virioplankton communities in surface and bottom waters. (A) Virioplankton PFGE fingerprint. (B) Autoradiogram of panel A probed with RAPD 1. (C) Autoradiogram of panel A probed with RAPD 2. Lanes: 1, molecular size markers of lambda phage concatamers; 2, 108 PFU each of genomic DNAs from three Chesapeake Bay bacteriophages; 3, virioplankton PFGE of water sample from station 834 in June 1996; 4, virioplankton PFGE of water sample from station 834B in June 1996; 5, 6, and 7, virioplankton PFGE of water samples at various depths from station 818 in July 1996.

Article Snippet: Virioplankton plugs and plugs containing phage lambda concatamers (Promega, Madison, Wis.), serving as molecular size markers, were placed into wells of a 1% SeaKem GTG agarose (FMC) gel with an overlay of molten 1% agarose.

Techniques:

Journal:

Article Title: Hybridization Analysis of Chesapeake Bay Virioplankton †

doi:

Figure Lengend Snippet: Conceptual model of virioplankton regulation of host community diversity. For each phage-host system, a selective factor stimulates growth of a specific host. An epidemic of phage infection begins at a critical threshold host cell density, and the abundance of a specific phage increases; thereafter, phage lysis causes the abundance of host cells to decline to background levels, preventing overdominance of a single host species. At the end of the epidemic, numbers of infective phage decline to a baseline level at a decay rate specific for each phage. It is also possible that the phage-host systems are temperate. Stimulation of host growth by a selective event causes curing of lysogeny and thus a release of phage. While abundances of specific hosts and phages change rapidly, the overall abundance of virio- and bacterioplankton is stable over longer, seasonal scales. A and D, moderate burst size of 10 to 50; B and C, large burst size of 100 to 500; A and B, low decay rate; C and D, high decay rate.

Article Snippet: Virioplankton plugs and plugs containing phage lambda concatamers (Promega, Madison, Wis.), serving as molecular size markers, were placed into wells of a 1% SeaKem GTG agarose (FMC) gel with an overlay of molten 1% agarose.

Techniques: Infection, Lysis